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mouse anti pp53 s15  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc mouse anti pp53 s15
    Mouse Anti Pp53 S15, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 3427 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+pp53+ser15/Phospho-p53+(Ser15)+Antibody/pmc13039127-214-11-15
    Average 96 stars, based on 3427 article reviews
    mouse anti pp53 s15 - by Bioz Stars, 2026-11
    96/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Reactive nucleolar and Cajal body responses to proteasome inhibition in sensory ganglion neurons.
    Article Snippet: Mouse monoclonal antibodies anti-UBF (F-9) (Santa Cruz Lab. La Jolla, CA, USA, dilution 1:100 IF), anti-SMN (BD transduction laboratories, dilution 1:50 IF), anti-fibrillarin (Abcam, dilution 1:500 IF), anti-p53 (Pab 246) (Santa Cruz Lab. La Jolla, CA, USA, dilution 1:500WB) and anti-B23 (Abcam, dilution 1:200 IF). .. Rabbit polyclonal antibodies anti-coilin (210.4) (dilution 1:200 IF), anti-fibrillarin ([41], dilution 1:100, immunogold), anti-Pp53 (Ser15) (Cell Signaling, dilution 1:1000 WB) and anti-Myc (Santa Cruz Lab. La Jolla, CA, USA, dilution 1:1000 WB). .. Goat polyclonal antibody anti-actin (Santa Cruz Lab. La Jolla, CA, USA, dilution 1:2000 WB).

    other:

    Article Title: Chemotherapy enhances HMGA1 secretion through the mutant p53-CK2 axis in pancreatic ductal adenocarcinoma cells
    Article Snippet: The pellet of extracellular proteins was resuspended in 150 μL of RIPA buffer (150 mM NaCl, 50 mM Tris-HCl pH 8, 1% IGEPAL, 0.50% NaDoc, 0.1% SDS) with 1X Halt protease and phosphatase inhibitor cocktail (Thermo Scientific, 1861284) and quantified using the Bradford Protein Assay Kit (Thermo Scientific, 1856209) according to the manufacturer’s instructions.

    Cell-Signaling:

    Article Title: Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.
    Article Snippet: Overexpression of the ubiquitous type II melanoma antigen-D2 (MAGED2) in numerous types of cancer suggests that this protein contributes to carcinogenesis, a well-documented characteristic of other MAGE proteins.. Modification of MAGED2 intracellular localization during cell cycle phases and following treatment with camptothecin (CPT) and phosphorylation by ATM/ATR following ionizing irradiation led us to investigate the molecular functions of MAGED2 in the cellular response to DNA damage.. Cell cycle regulators, cell cycle progression, and bromodeoxyuridine (BrdU) incorporation were compared between MAGED2-sufficient and -depleted U2OS cells following exposure to CPT.

    Article Title: Urodele p53 tolerates amino acid changes found in p53 variants linked to human cancer
    Article Snippet: Proteins were then transferred onto polyvinylidene difluoride membranes (Immobilon-P, Millipore, Bedford, MA). .. Anti-Pp53 ser15 (#9284, Cell Signaling technology, MA) and Anti-p53 CM5 (NCL-p53-CM5p, Novo Castra laboratory, UK) rabbit affinity isolated antibodies were used to detect axolotl p53. .. Immunodetection of primary antibodies was visualized using the ECL Western blotting kit (Amersham Pharmacia, Amersham, United Kingdom) or Lumi-light Western Blotting Substrate (Roche) following manufacturer's guidelines.

    Isolation:

    Article Title: Urodele p53 tolerates amino acid changes found in p53 variants linked to human cancer
    Article Snippet: Proteins were then transferred onto polyvinylidene difluoride membranes (Immobilon-P, Millipore, Bedford, MA). .. Anti-Pp53 ser15 (#9284, Cell Signaling technology, MA) and Anti-p53 CM5 (NCL-p53-CM5p, Novo Castra laboratory, UK) rabbit affinity isolated antibodies were used to detect axolotl p53. .. Immunodetection of primary antibodies was visualized using the ECL Western blotting kit (Amersham Pharmacia, Amersham, United Kingdom) or Lumi-light Western Blotting Substrate (Roche) following manufacturer's guidelines.



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    Image Search Results


    Mechanisms of PD-L1 regulation in lung cancer. PD-L1 expression in tumor cells is regulated at multiple levels. The p53–p21 axis can negatively influence PD-L1 transcription, while oncogenic pathways such as PI3K/AKT and JAK/STAT promote its up-regulation. Epigenetic mechanisms play a critical role: histone acetylation and methylation dynamically regulate chromatin accessibility, and DNA methylation (e.g., via DNMT1) can repress or activate PD-L1 transcription depending on context. Collectively, these mechanisms contribute to PD-L1-mediated immune evasion and tumor survival.

    Journal: Cancers

    Article Title: Regulation of PD-L1 Expression by SAHA-Mediated Histone Deacetylase Inhibition in Lung Cancer Cells

    doi: 10.3390/cancers17172919

    Figure Lengend Snippet: Mechanisms of PD-L1 regulation in lung cancer. PD-L1 expression in tumor cells is regulated at multiple levels. The p53–p21 axis can negatively influence PD-L1 transcription, while oncogenic pathways such as PI3K/AKT and JAK/STAT promote its up-regulation. Epigenetic mechanisms play a critical role: histone acetylation and methylation dynamically regulate chromatin accessibility, and DNA methylation (e.g., via DNMT1) can repress or activate PD-L1 transcription depending on context. Collectively, these mechanisms contribute to PD-L1-mediated immune evasion and tumor survival.

    Article Snippet: pp53 (Phospho-p53) , CST , 12571 , 1:1000.

    Techniques: Expressing, Methylation, DNA Methylation Assay